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Representatives sought for HIV/AIDS immune status or CD4 levels test

HIV/AIDS immune status or CD4 levels test

Available from Rapid Technologies, +2711 880 0364, a one-step screening test for the semi-quantitative determination of immune status in HIV/AIDS patients via detection of specific proteins from activated C cells that correlate to CD4 counts (CD4 Equivalents) using whole blood samples, serum or plasma.

The test was designed for the semi-quantitative determination of immune status in HIV/AIDS patients under conditions where such testing methods are not available or is cost prohibitive especially in resource poor and or rural settings.

AIDS is characterised by changes in the amount of T-cell lymphocytes. The virus in infected individuals causes a depletion of the T-helper cells, which are a sub population of T-cells. This leaves patients susceptible to opportunistic infections and potential malignancies. The presences of the virus itself cause the immune system to deteriorate as AIDS progresses. In normal and immune suppressed individuals there are specific proteins that are detectable that correlate to CD4 counts and which can be detected by the CD4. Tests such as CD4 counts are among the most widely used method for determining the immune status of HIV/AIDS of infected patients and to establish the efficacy of and or timing of the start of ART (anti-retroviral therapy) and or HAART (highly active anti-retroviral therapy)

The test is a rapid membrane based screening test to detect the presence of specific proteins that correlate to CD4 counts. This test is the newest generation lateral flow immunochromatographic type assay. These are among the simplest and easiest to use POC (point of care) assays requiring no instrumentation or highly skilled individuals to perform.

The test can be used with fresh EDTA anti-coagulated whole blood. The test employs the use of antigen binding monoclonal antibody conjugated to a colloidal gold particle and a unique combination of monoclonal antibodies immobilised on the membrane.

Once the sample is added to the tape cassette, the whole blood is separated allowing the plasma to pass and the antigen binding/bold complex, which then binds the specific target protein in the sample. As this complex passes over the immobilized antibodies on the membrane, if any specific CD4 equivalent proteins are present the antibodies capture them in turn. This produces a pink/purple band in the C area. This control band indicates that the test has been performed properly.

Kit Components

Each test kit contains:
1. HIV/AIDS immune status or CD4 test packs-25
2. 25 Plastic sample pipets (one pipet inside each test pouch)
3. Dropper vial Sample Pad Preparation Reagent
4. Directions for usage

Stability and Storage Conditions

The tests are stable at any temperature between 8-30’C when in the unopened pouches.
Stability of the kit is 24 months from the date of manufacture- dating is indicated on the pouch and kit label


For IN VITRO Diagnostic Use.

More info contact Suza Adam at suza.adam @ gmail.com

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